DNA fromAspergillus flavuscontains 5-methylcytosine
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چکیده
منابع مشابه
Oxidative damage to 5-methylcytosine in DNA.
Exposure of pyrimidines of DNA to ionizing radiation under aerobic conditions or oxidizing agents results in attack on the 5,6 double bond of the pyrimidine ring or on the exocyclic 5-methyl group. The primary product of oxidation of the 5,6 double bond of thymine is thymine glycol, while oxidation of the 5-methyl group yields 5-hydroxymethyluracil. Oxidation of the 5,6 double bond of cytosine ...
متن کاملIdentifying 5-methylcytosine and related modifications in DNA genomes.
Intense interest in the biological roles of DNA methylation, particularly in eukaryotes, has produced at least eight different methods for identifying 5-methylcytosine and related modifications in DNA genomes. However, the utility of each method depends not only on its simplicity but on its specificity, resolution, sensitivity and potential artifacts. Since these parameters affect the interpret...
متن کاملOxidatively generated damage to DNA at 5-methylcytosine mispairs.
Oxidatively generated damage to DNA has been implicated as causing mutations that lead to aging and disease. The one-electron oxidation of normal DNA leads to formation of a nucleobase radical cation that hops through the DNA until it is trapped irreversibly, primarily by reaction at guanine. It has been observed that 5-methylcytosine (C(m)) is a mutational "hot-spot". However, C(m) in a Watson...
متن کاملQuantification of 5-methylcytosine in DNA by the chloroacetaldehyde reaction.
The study of changes in genome-wide levels of DNA methylation has become a key focus for understanding the epigenetic regulation of gene expression. Many procedures exist to study DNA methylation, falling into two categories: gene-specific and genome-wide. Genome-wide methylation analysis is best performed by DNA hydrolysis followed by HPLC; however, it requires access to an HPLC machine, which...
متن کاملInhibition of human O6-methylguanine-DNA methyltransferase by 5-methylcytosine.
The ability of cloned human O6-methylguanine-DNA methyltransferase to repair a methylated guanine in a CpG-containing sequence, i.e., island, was studied by using a synthetic double-stranded 20-mer oligonucleotide from codon 248 of the p53 gene and another designed sequence. The double-stranded oligonucleotides incorporating 5-methylcytosine (5mC) and O6-methylguanine (O6mG) in various combinat...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2001
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2001.tb10939.x